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UCL Home  /  Geography  /  Resources  /  Laboratory  /  Light Microscopy  /  When to use dark field illumination

When to use dark field illumination

Dark field illumination is most readily set up at low magnifications (up to 100x), although it can be used with any dry objective lens. Any time you wish to view everything in a liquid sample, debris and all, dark field is best. Even tiny dust particles are obvious. Dark field is especially useful for finding cells in suspension. Dark field makes it easy to obtain the correct focal plane at low magnification for small, low contrast specimens.